Polyacrylamide brush layer for hydrophilic interaction liquid chromatography of intact glycoproteins
نویسندگان
چکیده
منابع مشابه
Characterization of intact neo-glycoproteins by hydrophilic interaction liquid chromatography.
In this study, an HPLC HILIC-UV method was developed for the analysis of intact neo-glycoproteins. During method development the experimental conditions evaluated involved different HILIC columns (TSKgel Amide-80 and ZIC-pHILIC), and water-acetonitrile mixtures containing various types of acids and salts. The final selected method was based on a TSKgel Amide-80 column and a mobile phase compose...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2013
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2013.05.076